Bus
Take the bus 720255 at the station
The journey takes approximately 4 h 2 min. Ticket price is about 13–18 EUR. The travel distance is 196 km.
Carrier is Tour Bus.
Get off at the České Budějovice
Train
Take the train 652 at the station Brno Hlavni Nadrazi .1
The journey takes approximately 4 h 14 min. Ticket price is about 10–18 EUR. The travel distance is 231 km.
Carrier is Czech Railways (ČD).
Get off at the Ceske Budejovice
Drive
In a rental car
The drive will take 2 h 19 min.. The distance between Brno and Ceske Budejovice is 216 km. You will use 17 liters of gasoline at a cost of €22.1.
See on this site which rental company has the best deal.